Imaging Core

 


The VGTI Florida® Imaging Core is housed in room 104 and features the following instrumentation:

1)    Olympus fluorescent microscope for immunohistochemistry and fixed sample standard fluorescent microscopy.

2)    Applied Precision Delta Vision Elite Imaging System for 7-color fluorescent confocal microscopy with live cell imaging capability featuring a CO2/heated chamber.

The DeltaVison imaging system is a high throughput microtiter plate based wide field deconvolution restoration microscope. It is built on an Olympus IX71 inverted microscope with a 250 watt xenon lamp and a LED transillumination light source. The NanoMotion III microtiter plate stage can hold multiple format plates (96-, 384- and 1536 well microtiter plates) as well as traditional slides.

Four objectives (10X, 20X, 40X and 100X) are supplied with the DeltaVision Core for a wide range of optical imaging needs. The system contains a 10 position emission module and a 7 position eyepiece module for wide range of fluorescent dyes. The addition of the environmental chamber allows time-lapsed live cell imaging. Microscope capabilities include Z-sectioning and 3D reconstruction, time series capture for following cellular events, determining co-localization of multiple proteins and/or dyes.

Microscopy training is available as well as imaging experimentation consultation with our dedicated core staff.  All microscopes are available for use through an online calendar reservation system and certified, trained individuals can use the scope on a fee for service basis either independently or with technical assistance by the core staff.

Please contact the following for more information:

Director of Scientific Operations:
John Schatzle Ph.D.
Phone: (772) 345-5718
Email:
Sr. Research Assistant:
Kim Kusser
Email: